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eEnzyme/Total ROS Activity Assay Kit (Green Fluorescence)/CA-R900/1 Ea
来自 : 发布时间:2024-05-04

CA-R900

Name

EliteTM Intracellular Total ROS Activity Assay Kit (Green Fluorescence)

Description

This kit uses a unique ROS sensor to quantify ROS in live cells. Elite™ ROS Green is cell-permeable. It generates the green fluorescence when it reacts with ROS. The kit is an optimized \"mix and read” assay format that is compatible with HTS liquid handling instruments.

Application

The kit provides a sensitive one-step fluorimetric assay to detect intracellular ROS (especially hydrogen peroxide) in live cells with 1 hour incubation.

Size

200 assays (or two 96-well plates)

Detection

Fluorescence microplate reader (Ex/Em = 490/525 nm)

Click here forDATA SHEET

Click here for MSDS

Users Guide for EliteTM ROS Activity Assay Kits

CA-R900

CA-R911

CA-R922

CA-R933

Ex/Em (nm)

490/525

520/605

540/570

650/675

Fluorescence Signal

Green (green)

Red (red)

Orange (orange)

Deep Red (deep red)

Target ROS

Hydrogen peroxide

superoxide

superoxide, hydroxyl radicals

superoxide, hydroxyl radicals

Application

total

total

mitochondria

mitochondria

Signal Strength

++++

+++

++++++

+++++

Detection

1,2

1,2

1,2,3

1,2,3

*1-fluorescence microplate reader; 2- fluorescence microscope; 3- flow cytometry

本文链接: http://eenzyme.immuno-online.com/view-1460627452.html

发布于 : 2024-05-04 阅读()
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